IUBio

protein disulfide bonds' trapping and quantification

Frank Fürst ffrank at rz.uni-potsdam.de
Mon Aug 5 12:28:37 EST 2002


zhang at mpimp-golm.mpg.de schrieb:

> Who can help to provide a reliable protocol?

For the task in the subject: protein disulfide bonds' trapping and
quantification? 

Have a look at Creighton-TE, J. Mol. Biol. 113 (1977), 295-312

or probably newer texts that cite this, or by Creighton in one of his
books: Protein Function/Protein Structure.

Ah, there it is: 

Creighton, TE (ed.), Protein Structure, A Practical Approach,
Oxford University Press, NY 1997

especially therein:
Hollecker, Michelle, Counting integral numbers of residues by chemical
modification, section 3: Disulfide bonds (pp. 155-159)

and

Gray, William R., Disulfide bonds between cysteine residues, pp. 165-186

HTH, Frank
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