Dear colleagues,
I have problem in purifying my protein of interest from the GST from
using GST column. I have cut my protein of interest (~25.7kDa) from the
GST fusion protein (26 kDa) with thrombin protease, and as in theory, I
should get my protein of interest in flow through, but it didn't. I
found that it came out together with the GST when I eluted with the GST
elution buffer. Please give some suggestion to purify my protein of
interest from the GST protein. Thanks.
Joyce
