why don't you try to delete six nucleotides from the 5' of DNA, then purify
the mutant protein?
good luck
yong
""Lipari, Francesco"" <Francesco.Lipari at DSM.COM> wrote in message
news:896508DC6B0ED511A90400508B6838EE48DFDB at camnt11ntms002.mnt11.dsm-group.c
om...
> Hi,
> I am trying to remove two amino acids from the N-terminus of my favorite
> protein using aminopeptidases. The sequence of the protein is XaaXaaPro...
>> Apparently, it will be difficult to perform this because commercial
> aminopeptidases do not cleave at the Xaa-Pro bond.
>> I am looking for a solution to this very interesting problem.
>> Thank you,
>> Francesco Lipari, Ph.D.
> Senior Scientist Analytical Development
> DSM Biologics
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