Hi all!
How do you measure the amount of protein from expression studies? We've
been trying to quantify the yield from protein gels and from Western
blots, but without much success. The protein is denaturated from
inclusion bodies, and what we see is more of a smear than distinct
bands. So perhaps our method for preparing the samples are flawed. Do
you know of any good protocols for preparing pellet fractions containing
inclusion bodies that shall be run on gels and blotted? Does it happen
that not all of the inclusion bodies are avaiable for antibody binding
and hence detection?
The software is not helping either. We currently use Bio-Rads Quantity
One software, do you know of any other software that may do the trick,
that is to quantify bands from either gel or blot and estimate yield
compared to a standard?
Kind regards,
Trond Erik Vee Aune
http://kibt.chembio.ntnu.no/molgen/
