denaturing acid gel, non-reducing conditions

Frank Fürst ffrank at rz.uni-potsdam.de
Tue Nov 13 13:32:14 EST 2001

We are thinking of monitoring disulfide linkage of an oligomeric protein
by quenching disulfide exchange under acid conditions and running a
denaturing, but non-reducing electrophoresis gel afterwards. 

Has anybody experience in running (SDS-)gels under acid conditions, or
can recommend some literature?

Thanks, Frank

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