6His co-eluted protein ?

Emir Khatipov khatipovNO at NOuchicago.edu
Fri Nov 9 13:08:47 EST 2001

Looks like P30856 in your list  is the proline isomerase (aka FKBP-TYPE
RICH PROTEIN) (WHP) - according to NCBI).

- Emir

"Michael Witty" <mw132 at mole.bio.cam.ac.uk> wrote in message
news:Pine.SGI.4.33.0111091349440.991273-100000 at mole.bio.cam.ac.uk...
> Dear Emir,
>          I went to the trouble and expense of having some of these
> proteins that co-purify with His tagged proteins identified by N-terminal
> sequencing (my dream was that one of them would be binding to the protein
> I was purifying, which is part of a phage receptor and we suspect it of
> binding to _something_).
> What I got was
> Protein  Name
> P04475.  HSP70
> P77398   Methyltransferase?
> P17169   Glucosamine-6-P Synthase (PDB file 1GDO).
> P30856   FK506-binding protein
> P03020   cAMP receptor
> All very histidine rich, and made a nice slide for a lab talk, but with no
> significance to phage infection of E. coli or anything else.
> I will add proline isomerase to my list.  Do you have an accession number
> or a amino acid sequence for proline isomerase?
>                                               Mike.

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