6His co-eluted protein ?

Michael Witty mw132 at mole.bio.cam.ac.uk
Fri Nov 9 09:04:11 EST 2001

Dear Emir,
         I went to the trouble and expense of having some of these
proteins that co-purify with His tagged proteins identified by N-terminal
sequencing (my dream was that one of them would be binding to the protein
I was purifying, which is part of a phage receptor and we suspect it of
binding to _something_).

What I got was

Protein  Name
P04475.  HSP70
P77398   Methyltransferase?
P17169   Glucosamine-6-P Synthase (PDB file 1GDO).
P30856   FK506-binding protein
P03020   cAMP receptor

All very histidine rich, and made a nice slide for a lab talk, but with no
significance to phage infection of E. coli or anything else.

I will add proline isomerase to my list.  Do you have an accession number
or a amino acid sequence for proline isomerase?

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