IUBio

SPS-PAGE loading control protein

D.K. dk at no.email.thankstospam.net
Thu Nov 1 07:59:58 EST 2001


In article <9rp47o$150u$1 at news.net.uni-c.dk>, "Silas Bruun" <Silas at knus.dk> wrote:
>Hi All
>I'm looking for an antibody that I can use for loading control. The problem
>is that it should have somewhat high molecular weight. Therefore Actin and
>GAPDH are out of the question.
>
>I've been suggested to use MCM7, but I'm not sure whether it is usable as
>such a control (I havent seen it in any papers).
>
>Do you have any other suggestions for such a control with a weight of >60 kD
>
>cheers
>silas
>

How about Hsp90 (90K, present n all cells, very abundant)?
If really high is desired, myosin do. 

DK





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