protein folding

ChenHA hzhen at freeuk.com
Sat May 12 16:07:18 EST 2001

"Inés Baeza Hernández" wrote:
>    Dear colleagues.
> I'm working in overexpression of an halophilic protein in E.coli. I obtain
> this protein as inclusion bodies, but I cannot refolding it. I have proven
> several buffers (tris, phosphate, hepes) at different pH, salt and
> temperature. Have you some idea?. Thanks in advance

There are large number of protocols for refolding proteins, I
would therefore suggest that you read a few review articles on
this subject.  If you need references let me know because I need
to dig them out.  One protocol you can find online is useful:


Note that there is no guarantee that a protein can be correctly

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