"Inés Baeza Hernández" wrote:
>> Dear colleagues.
> I'm working in overexpression of an halophilic protein in E.coli. I obtain
> this protein as inclusion bodies, but I cannot refolding it. I have proven
> several buffers (tris, phosphate, hepes) at different pH, salt and
> temperature. Have you some idea?. Thanks in advance
There are large number of protocols for refolding proteins, I
would therefore suggest that you read a few review articles on
this subject. If you need references let me know because I need
to dig them out. One protocol you can find online is useful:
http://www.path.cam.ac.uk/~lv213/
Note that there is no guarantee that a protein can be correctly
refolded.
