FPLC systems

Frank Fuerst ffrank at rz.uni-potsdam.de
Fri May 11 09:57:59 EST 2001

Geoff Briggs <geoff.briggs at nottingham.ac.uk> wrote:

>Having just changed labs, I'm not sure what version I was
>using - I think it was version 2.1. 

We use 2.0, which might account for the instability, since
x.0-versions tend to be "immature".

>which did annoy me were the long boot-up times, and the fact
>that it was only supported on a win 95a system - not even a
>98 version. 

I don't prefer Win98 over 95, but what I want is a real operating
system, e.g. Windows NT /2K. Unfortunately there is no mention of NT
on the disk (but it starts installing itself on NT, as I just tried).
Does anybody know if it works?

>It's file handling could be better too.


>However, as for the machine itself; I liked 
[lots of things]

>Of course, most of
>these (all?) 

all (but I'm not sure with Excel export)

>are available on the Akta, but at a much higher
>price, and none were available on the old FPLC systems,
>which is what I have to use in my new job :-(

Yes, for that price it's really okay.

But one thing that does annoy me very much is the limited possibility
for scaling graphs. If you elute with a buffer that has some
208nm-absorption and want to look at trace peaks over a buffer
baseline of, say, 0.3 OD, you run into problems. You can scale from 0
to 0.5, and you'll see your gradient (and major peaks of course), but
not small peaks, or from 0 to 0.25 (don't remember the exact limits,
just an example), and you'll not see anything, because the baseline's
over 0.25. What I would like is 0.25 to 0.35, but that is not

Bye, Frank

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