Try running a decreasing gradient of urea 8 to 0M over say 5-10 column volumes
before you elute your protein from the Ni-NTA column. I hear this results in
pretty good folding but haven't tried it personally...
Bull Zebra wrote:
> I am purifying a 6XHis-tagged protein under denature condition (8M urea)
> using Ni-NTA agarose and I need to renature the protein in order to couple
> it to an affinity column (CNBr method). Anybody there can suggest a good
> renaturation protocol for this purpose? Thanks in advance!
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