Talon resin histidine use

Dr. Artem Evdokimov eudokima at mail.ncifcrf.gov
Tue May 1 13:04:51 EST 2001

Can you use nickel to elute your protein ?


John Eisses wrote:
> Bionetters,
> I am purifying a delicate membrane protein and imidazole seems to
> inhibit it, but when I use histidine, the Talon (Co2+) resin bleaches
> out and the buffer turns brown. Are there alternatives for imidazole? I
> seem to remember a ref for N acetyl Histidine , but does this strip the
> Talon resin? I'll try buffer exchange after the fact but I'd like to not
> lose activity as my experience is once its gone it doesn't come back.
> salud
> Ted Michelini
> michelini at ohsu.edu

|Dr. Artem Evdokimov   Protein Engineering |
| NCI-Frederick        Tel. (301)846-5401  |
|              FAX (301)846-7148           |
|        eudokima at mail.ncifcrf.gov         |
|      http://www.ncifcrf.gov/plague       |

More information about the Proteins mailing list

Send comments to us at biosci-help [At] net.bio.net