Bionetters,
I am purifying a delicate membrane protein and imidazole seems to
inhibit it, but when I use histidine, the Talon (Co2+) resin bleaches
out and the buffer turns brown. Are there alternatives for imidazole? I
seem to remember a ref for N acetyl Histidine , but does this strip the
Talon resin? I'll try buffer exchange after the fact but I'd like to not
lose activity as my experience is once its gone it doesn't come back.
salud
Ted Michelini
michelini at ohsu.edu
