IUBio

culture contamination

maya maya-khorchide at bigfoot.de
Sun Mar 25 04:54:26 EST 2001


I am working with cell cultures and it seems that I have a contamination
problem.
Under light microscopy dark dots are visible in the cells and are likely
to be also moving on their surface. Cell growth, attachment and
viability are affected.
These mysterious creatures are only observed in association with cells
but not in incubated cell free medium.
Years ago only some cultures were involved but now the infection is
worsening and spreading about other cultures.
If you have an idea who these intruders could be or have an advice how
to recognize them or get rid of them please help me.

MAYA




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