dsummers at mtcnet.net (Dana Summers) wrote:
>6. Saturate to 0.7 saturation. How many grams of ammonium sulfate do
> I add to 1 liter of solution?
442g at 0°C, 470 at room temperature, data taken from a protein
methods lab book - I think it's called "Labfax"
>7. Dissolve the precipitate from 0.7 saturation in 0.25 saturated
> ammonium sulfate. How many grams of ammonium sulfate do I add to
> make a 0.25 saturated ammonium sulfate if I have 10 grams of precipt?
I usually neglect the ammonium sulfate in the pellet, just cleanly
discard the supernatant and add 0.25 saturated solution (144g/l at RT)
>8. Adjust pH to 5.0.
>9. Let stand 2 days at 25 degrees C.
>10. Filter.
>11. Recrystallize the crystals on the filter pad in 0.25 saturated
> ammonium sulfate at pH 5.0 about 8 times. How many grams of
> ammonium sulfate do I add to make a 0.25 saturated ammonium
>sulfate if I have 10 grams of crystals?
Here clearly you can neglect AS in the crystal suspension, just work
with 25% AS in the solvent.
I don't have tables for MgSO4, sorry.
Bye, Frank
