IUBio

help pls -> Masson trichrome stain

M. Hertel hippocampus at NoSpam.gmx.ch
Fri Mar 9 01:34:04 EST 2001


hello Jingjing
In tissue sections? Not with Ab's?
There is a protocol to stain extra cellular matrix, especially collagen,
trichrome stain after Masson: nuclei are blue-black, cytoplasm, muscle
and ceratins are red and collagen in blue-green. The protocol I have is
in german, so I checked the net there is a protocol in english under:
http://www.members.pgonline.com/~bryand/stain/conektv/masson.htm
looking quite similar to ours
If you use   www.google.com   and the search string "trichrome, masson"
you will find even more, also combinations with other stains for elastic
fibres etc.
good luck
Moritz

"Bi, Jingjing" wrote:

> Hi all,
> Is there any protocols to stain proteins like fibronectin or laminin?
> Apreciate your help.
> Jingjing






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