In article <3AA37B33.B1AF72B0 at unix.ccc.nottingham.ac.uk>, the eminent
Kevin Brady at Organic Chemistry, Nottingham wrote
> When I run fractions out on an SDS-PAGE gel, I can see the 37kDa
> band, but also about 6 smaller proteins ranging from about
> 35-22kDa. These dont appear in the wash steps at all, and are
> specifically eluted with GSH. I'm using BL21(DE3)pLysS E.coli with
> pET42b for expression. Has any one else seen these sorts of
> proteins appearing?
Proteolysis of the fusion during expression?
Duncan
--
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.comhttp://www.genesys.demon.co.uk
