How do I extract free amino acids form leaf tissue?

Lee Hunt lee.hunt at which.net
Fri Jun 29 13:55:58 EST 2001

I used to desalt with Sephadex G-25, although this can result in a

Tony Larson wrote:

> I'd like to know a method to extract free amino acids from small
> (5-100 mg) amounts of plant leaf tissue.  These will than be
> derivatized and analyzed by either HPLC or GC.  I would particularly
> like to know the best way to clean up the extracts, to remove
> background contaminants, in as small a working volume as possible
> before derivatization.  This is because I will need to process a large
> number of samples.

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