I used to desalt with Sephadex G-25, although this can result in a
dilution
Tony Larson wrote:
> I'd like to know a method to extract free amino acids from small
> (5-100 mg) amounts of plant leaf tissue. These will than be
> derivatized and analyzed by either HPLC or GC. I would particularly
> like to know the best way to clean up the extracts, to remove
> background contaminants, in as small a working volume as possible
> before derivatization. This is because I will need to process a large
> number of samples.