Frank at Fackelmayer.de wrote:
::Dima Klenchin wrote:
:> :They have the transfer running with a current of 0.65 mA/sq. cm. of gel
:>:> That would be per sq.cm of gel's cross-section (width x thickness),
:> not just "gel".
:>::Hmmm, not sure. We always use 0.8-1mA per sq.cm of gel (width x height),
:and I wouldn´t really understand what the thickness of the gel would
:have to do with it. Of course, a thicker gel takes somewhat longer to blot.
True. What I originally wrote is, of course, rubbish.
For some reason, I has not read (?) the sentence I replied
to and been thinking about electrophoresis, not
electrotransfer. Ugh.
- Dima
