Western blues

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Tue Jun 26 20:59:37 EST 2001

Frank at Fackelmayer.de wrote:
:Dima Klenchin wrote:
:> :They have the transfer running with a current of 0.65 mA/sq. cm. of gel
:> That would be per sq.cm of gel's cross-section (width x thickness),
:> not just "gel".
:Hmmm, not sure. We always use 0.8-1mA per sq.cm of gel (width x height),
:and I wouldn´t really understand what the thickness of the gel would
:have to do with it. Of course, a thicker gel takes somewhat longer to blot.

True. What I originally wrote is, of course, rubbish. 
For some reason, I has not read (?) the sentence I replied
to and been thinking about electrophoresis, not
electrotransfer. Ugh.

        - Dima

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