> :Dima,
> : here is how it might happen if the stack of paper towels makes good
> :contact with the buffer reservoir rather than just touching the gel. You
> :have to position the papers towels _really_ badly. Mike.
> :
> : ---------------------------------
> : -- > --------------------------- Badly positioned stack of gels
> : - / -----------------------------
> : I IIIIIIIIIIIIIIIIIIIIIIIIII Gel
> : I B U F F E R
> :Path of
> :short
> :circuit
>> ^ ^ ^
> | | |
>> Here here and here
> (no gel or towels)
>> current will be the same.
>> As far as I can imagine, conductivity of buffer and towels
> and porous gel wetted in that buffer are essentially equal.
> In this case, you'd have to have that "badly positioned stack
> of towels" to be of enormous thickness or insert an insulator
> between gel and paper (air bubble, for example) to have
> the current bypass the gel.
I have feeling we are at crossed purposes: I am describing a blot with a
bunch of towels sucking up buffer by capillary action. I think you are
describing electroblotting. Am I right? Mike.
