IUBio

electrophoresis 2D

Mitchell Isaacs misaacs at spam_me_not.student.usyd.edu.au
Fri Jun 22 21:40:59 EST 2001


"Martin Benoît" <benoit.martin at bordet.be> wrote in message
news:DF1E44F5DBD2D211B2100060087E02B13F6AF9 at mail.bordet.be...
> Hi everybody,
>
> I am now performing electrophoresis 2D on plasma. The gels were stained
with
> silver staining
> I would like to know if somebody knows a method to quantify each protein
in
> the gel 2D.
> Is it possible to correlate the optic density and the protien
concentration
Not with silver - you want coomassie or cypro Ruby.

Ruby is the best - most sensitive & quantitative over the widest range.

Cheers,
Mitchell





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