Tryptophan from PAGE gel hydrosylate

Mitchell Isaacs misaacs at spam_me_not.student.usyd.edu.au
Mon Jun 11 21:00:34 EST 2001

"ckf" <craig at fulton52.freeserve.co.uk> wrote in message
news:9g2vaf$i26$1 at news5.svr.pol.co.uk...
> jose
> I read your method for trypsin digests of PAGE samples. Is the method (and
> subsequent reverse phase HPLC) compatible with SDS from denaturing gels or
> is the method only compatible with native PAGE
If you check out the technical bulletin for promega trypsin,
(http://www.promega.com/tbs/tb512/TB512.pdf) you will see that it is
resistant to mild denaturing conditions, eg 0.1% SDS.


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