Tryptophan from PAGE gel hydrosylate

jose jose at biochemistry.cwru.edu
Mon Jun 11 16:05:51 EST 2001

ckf wrote:

> I read your method for trypsin digests of PAGE samples. Is the method (and
> subsequent reverse phase HPLC) compatible with SDS from denaturing gels or
> is the method only compatible with native PAGE

i think i use it with SDS containing PAGE gels, too. it may be worth
your while to investigate the use of a heartier protease, one that is
SDS resistant, if you suspect thats a problem.

jose nazario				jose at cwru.edu

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