ckf wrote:
> I read your method for trypsin digests of PAGE samples. Is the method (and
> subsequent reverse phase HPLC) compatible with SDS from denaturing gels or
> is the method only compatible with native PAGE
i think i use it with SDS containing PAGE gels, too. it may be worth
your while to investigate the use of a heartier protease, one that is
SDS resistant, if you suspect thats a problem.
jose nazario jose at cwru.edu