his-tag buffer

Dr. Artem Evdokimov eudokima at mail.ncifcrf.gov
Tue Jun 5 15:22:22 EST 2001

> . . . I thought there was also some objection from the manufacturer
> because Tris can act as an electrophile.  However, my chemistry is very
> bad and I don't quite know how that can be bad.  Mike.

Tris is a primary amine. More likely to be a nucleophile (though this
would not matter).

|Dr. Artem Evdokimov   Protein Engineering |
| NCI-Frederick        Tel. (301)846-5401  |
|              FAX (301)846-7148           |
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