epitope tags and antibodies - your favorites?

Nancy Phillips phillinj at SLU.EDU
Fri Jun 1 19:46:22 EST 2001

hello netters, I want to confirm production of my gene-of-interest
transfected into human cancer cell lines. There are no specific antibodies
as of yet. I would like to make 5' and 3' epitope-tagged constructs, then
do westerns. I would also like to be able to purify said constructs on
affinity columns, to serve as blot controls. Eventually I would be doing
other things (pulldown expts, etc), but now I have very simple requirements.

What epitope tag and antibody combo (with clone name and supplier) has
worked well for you in Westerns?

Thanks for suggestions. I am new to this protein stuff.

Nancy Phillips, M.D.			phone:(314)577-8782
Pathology				fax:(314)268-5120
St. Louis University Hospital		email: phillinj at slu.edu
3635 Vista Ave.
St. Louis, MO, 63110, USA



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