hello netters, I want to confirm production of my gene-of-interest
transfected into human cancer cell lines. There are no specific antibodies
as of yet. I would like to make 5' and 3' epitope-tagged constructs, then
do westerns. I would also like to be able to purify said constructs on
affinity columns, to serve as blot controls. Eventually I would be doing
other things (pulldown expts, etc), but now I have very simple requirements.
What epitope tag and antibody combo (with clone name and supplier) has
worked well for you in Westerns?
Thanks for suggestions. I am new to this protein stuff.
Nancy Phillips, M.D. phone:(314)577-8782
St. Louis University Hospital email: phillinj at slu.edu
3635 Vista Ave.
St. Louis, MO, 63110, USA