Could you describe you rgel in more detail ? Is it a pre-cast gel or
did you pour it yourself ? What % AA ? What voltage/current ? For how
long ? What are you loading ? What are the buffers ?
A.
Amanda Cage wrote:
>> I have problems running SDS-PAGE gels because a pencil-like line runs
> continuously from one end of the gel to the other end of the gel
> horizontally at about 3/4 of the way down on the gel when I do the
> silver staining. I am not sure why that happens and do not know how
> to get rid of it. The strange thing is when I use commassie blue to
> stain a different gel (not the same gel as silver but contain the same
> samples), a color gradient appear across the gel. The top appear
> almost colorless. The middle of the gel appears a light blue. The
> bottom part of the gel (3/4 way down) appears a deeper blue than the
> middle. I could not get the entire gel to stain a uniform blue color.
> Could the problems with these two gels be the running buffer
> contaminants or is it something else? I washed the SDS off the gel
> very well so I believe that is not the problem. Please help! Thank
> you.
--
|Dr. Artem Evdokimov Protein Engineering |
| NCI-Frederick Tel. (301)846-5401 |
| FAX (301)846-7148 |
|eudokima at mail.ncifcrf.gov |
|http://mcl1.ncifcrf.gov/plague |
