What about guanidine hydrochloride ? As a salt, the guanidinium group is
very unreactive. Have you considered detergents (Tween, Triton, etc) or
perhaps nondetergent sulphobetaines ?
A.
Lee Hunt wrote:
>> I've purified a His- tagged protein from inclusion bodies using 6M urea.
> How can I then link it to affi-gel?. I've thought of dialysing but would
> prefer there to be a denaturant that doesn't have primary amine groups
> to replace the urea
--
|Dr. Artem Evdokimov Protein Engineering |
| NCI-Frederick Tel. (301)846-5401 |
| FAX (301)846-7148 |
|eudokima at mail.ncifcrf.gov |
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