Hello
I've been expressing His-tagged proteins in E. coli Origami cells, about 10
different proteins in all. I was using a His-probe for detection and was
getting only about half of the proteins expressed. Until I turned to
specific antibodies for detection eg, anti-prolactin and suddenly all
proteins were detected. All had been sequenced, the His-tag is 5` to the
protein sequence. Does anyone know why some are detected and others not by
the His-probe? This is a real problem because I had planned on using the
His-tag for purification.
Many thanks
Rick
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Richard J. Buick
E-mail: mail at richardbuick.com
URL: http://www.richardbuick.com
Tel(GSM): +44 7801 416792
"Light creates the shadows"
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