On Tue, 16 Jan 2001 14:42:23, "Randy" <rldurren at bchserver.bch.ncsu.edu> wrote:
ð Our lab is trying to find a procedure to purify Urea. Does anyone out there
ð have a procedure/reference to purify this chemical? Any help will be greatly
ð appreciated.
ð
For electrophoresis purposes, the only purification necessary is to remove
charged breakdown products like ammonia, etc. This is done by stirring the
solution of urea with mixed-bed ion exchange resin. Use a resin like BioRad AG
501-X8 (D) which contains a color indicator. Stir until the color changes from
blue to tan. Filter off the resin; repeat the process until the newly-added
resin doesn't change color.
For acrylamide gels, it's ideal to deionize the solution of acrylamide plus
urea, before adding buffer.
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