Hi Folks,
Has anyone come across a situation where a 6XHis tag has affected the
DNA-binding of a DNA-binding protein? (the pH is 8 so unlikely to be +ve
charge). I know the folding could be affected. Just wondered if anyone
had seen such a phenomenon...
Thanks,
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Dr. Matt Hicks
School of Biological Sciences
University of Sussex
Falmer
BN1 9QG
UK
Tel: +44(0)1273 678923
Fax: +44(0)1273 678433
email: matth at biols.sussex.ac.uk
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