IUBio

OD and IPTG concentration

Kyle Legate legatek at mcmail.cis.mcmaster.ca
Fri Dec 21 10:44:01 EST 2001


On Fri, 21 Dec 2001, Duncan Clark wrote:

> Wrong. It depends on what antibiotic is used. B-lactamases such as
> Ampicillin will be cleared in minutes but Neomycin and Kan will not. Why
> do you have to use antibiotics anyway? There are other ways of
> maintaining vectors than with antibiotics. In fact antibiotics are to be
> avoided if the end protein product is for in vivo use etc.
>
Please share some methods to maintain vectors without antibiotic. I always
thought that if the bug has no use for the resistance marker, it would
dump the plasmid, no exceptions.

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legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com

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