IUBio

Protein purification

Pier Carlo Montecucchi pcmontecucchi at libero.it
Thu Dec 20 09:40:05 EST 2001


Rick:

You could try isoelectric focusing (anal - prep)?  In some cases the two
protein forms (folded - unfolded) have different pI's.
Pier Carlo

Pier Carlo Montecucchi
Email: pcmontecucchi at compuserve.com




"Richard Buick" <Richard.Buick at btinternet.com> wrote in message
news:9vfusg$ni8$2 at knossos.btinternet.com...
> Hi
> I wonder if anyone could suggest a method for the separation of active
from
> inactive (mis-folded) protein. Affinity chromatography is not an option
> since the method must be applied to many different proteins. Ion exchange
> and hydrophobic interaction have been tried with not much success. The
> proteins to be separated are post IMAC.
> Thanks
> Rick
>
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> ***************************************************
> Richard J. Buick
>
> E-mail: mail at richardbuick.com
> URL: http://www.richardbuick.com
> Tel(GSM): +44 7801 416792
>
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> ***************************************************
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