Hi everyone!
While isolating inclusion bodies from E.coli for a expressed proteins
we are getting some black colored layer around the inclusion body
pellet.
It is not from the medium as the cell pellet is free from such color
and is normal whiteish brown. There seems to a difference in the
quantity of color formation depending upon the source of Yeast extract
and tryptone also.
I would like to know if any other group is also having similar results
and possible reasons for it and of course if it due to oxidation of
proteins how to get around such a situation
thanks,
S.Ballal
