HI everyone !
I would like to know how is the optimum concentration of IPTG used for
induction related to OD of the culture.
In most of the places varying concentration of IPTG has been suggested
for induction depending upon the expression system and other things.
Most of these are for an low ODs like 0.5-1.
In case we need to work on high ODs like 50-100 or 150 would one
require much higher concentration of IPTG and if in what range is
generally used for such cultures.
Thanks in advance
S.Ballal
Ahmedabad, India
