IUBio

Protein purification

Richard Buick Richard.Buick at btinternet.com
Sat Dec 15 11:48:17 EST 2001


Hi
I wonder if anyone could suggest a method for the separation of active from
inactive (mis-folded) protein. Affinity chromatography is not an option
since the method must be applied to many different proteins. Ion exchange
and hydrophobic interaction have been tried with not much success. The
proteins to be separated are post IMAC.
Thanks
Rick

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***************************************************
Richard J. Buick

E-mail: mail at richardbuick.com
URL: http://www.richardbuick.com
Tel(GSM): +44 7801 416792

"Light creates the shadows"
***************************************************


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--



***************************************************
Richard J. Buick

E-mail: mail at richardbuick.com
URL: http://www.richardbuick.com
Tel(GSM): +44 7801 416792

"Light creates the shadows"
***************************************************





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