Hi All
I have been doing the western Blots with the proteins from mammalin
cells. Interestingly, what I saw that after first western Blot, that I
did immidiately I got good bands. But after the storage of protein
extract for 7 to 8 days, I saw no bands at all, working with same
antibody and conditions. Predictably it is because of the degradation
of protein at the storage time. I kept it at -70 degree after the
solublisation with 5X sample buffer after the lysis and extraction of
the protein.
Can someone suggest the best storage condition for the protein before
and after the solublisation with sample buffer.
