Smeared Western Samples

axel siroy axel.siroy at wanadoo.fr
Wed Dec 5 16:03:16 EST 2001


what is the composition of the sample buffer (sophie) ?
I think U try to get a native protein by using triton in your extraction
(membrane proteins ?)but I was trying this detergent and it can trouble the
SDS-PAGE at high concentration (cmc ~0.025% for TX100).

like patrick, U can use Bmercaptoethanol with high [SDS] (1 or 2%) in the
sample buffer and try to heat the sample (with SB) 5 minutes at 100°C.

can U send me the results of these trials, for more explications

bye-bye, good job =0)


More information about the Proteins mailing list

Send comments to us at biosci-help [At] net.bio.net