In article <3AED5716.2A9525DF at mail.ncifcrf.gov>, "Dr. Artem Evdokimov"
<eudokima at mail.ncifcrf.gov> wrote:
> Actually filtering is necessary if you are working with any kind of FPLC
> or HPLC system, or using expensive fine-grain columns (such as the
> 'monoX' series, hydroxyapathite, 5u C-X and CN-X series, superdex etc.).
And I'd do it to protect the - not inexpensive - Ni-NTA resin.
> We are mostly dealing with proteins being prepared for crystallographic
> studies so as you can imagine, the amounts of liquids that we put
> through the systems are quite large, but even for small samples
> sometimes just one piece of fiber is enough to crack your column or
> increase the backpressure or get stuck in your pumps and
> cross-contaminate your sample.
And one piece of dust could give you a crystal you never see again . . .
BTW Michael, if you are doing this for crystallization and not getting
anywhere, you might try the same protein sans His-tag.
r
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