I am a nutritionist trying to prepare a fibrinogen solution (2g/L).
Problem is that it does not dissolve readily. It seems to stick
together. I have now added the fibrinogen powder carefully to the
phosphate buffered saline and just let it stay on top until it seems
dissolved - this is also not working well. Slowly mixing it leads to
aggregates, vigorous mixing is a disaster, ultra sound does not help,
neither does warming the buffer. Should I try using something else
rather than PBS (7.4)? Any suggestions will help
Johann Jerling
