In addition to Thiourea at 5mM use sodium metabisulphite (50mM or 100mM), PVPP 1% and beta mercaptoethanol (optional) 0.5%..
Good Luck
Savita
Gerardo Perez Quimica UNALCOL wrote:
> Hello Ligia:
> There is a procedure , which have worked well in our hands, described by :
> Cremer F., van de Walle C. in Anal Biochem . 147 : 22-26, 1985.
>> As polyphenols and other oxidable pigments are a nuisance we use thiourea
> to reduce the extent of oxidation. Have a look at :
> Van Driessche et al ., Anal Biochem., 141:184-188, 1984
> Good luck
>> Gerardo Perez
> ;
>> On Mon, 22 May 2000, llebron wrote:
>> > Hi there:
> > I worrk with coffee and the pathogen fungi Cercospora
> > coffeicola. I wonder to extract pathogenesis related
> > proteins from coffee leaves. Do anyone know a good protocol
> > for the extraction of leaves's proteins
> > Thanks in advance
> > Ligia
> >
> >
> > * Sent from AltaVista http://www.altavista.com Where you can also find related Web Pages, Images, Audios, Videos, News, and Shopping. Smart is Beautiful
> >
>> ---
