Hello Ligia:
There is a procedure , which have worked well in our hands, described by :
Cremer F., van de Walle C. in Anal Biochem . 147 : 22-26, 1985.
As polyphenols and other oxidable pigments are a nuisance we use thiourea
to reduce the extent of oxidation. Have a look at :
Van Driessche et al ., Anal Biochem., 141:184-188, 1984
Good luck
Gerardo Perez
;
On Mon, 22 May 2000, llebron wrote:
> Hi there:
> I worrk with coffee and the pathogen fungi Cercospora
> coffeicola. I wonder to extract pathogenesis related
> proteins from coffee leaves. Do anyone know a good protocol
> for the extraction of leaves's proteins
> Thanks in advance
> Ligia
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