I'm just running my second BN-page today! The blu is referred to Coomassie
blue that is used to charge proteins. I'm trying to use this technique to
study the equilibrium between dimers and tetramers in my proteins. These
are the references:
1. Schagger and von Jagow Blue Native Electrophoresis for Isolation of
Membrane protein complexes in enzymatically active form Analytical
biochemistry, 199, 223-231 (1991)
2. Schagger et al. Analysis of molecular masses and oligomeric states of
protein complexes by blue native electrophoresis and isolation of membrane
protein complexes by two dimensional native electrophoresis Analytical
Biochemistry, 217, 220-230 (1994)
The first time I've run it, I've not used the mini-gel but the 'big' gel
with some standards and my protein. Results were very good, mostly on some
of the proteins. Now I'm trying to do a more selective gel using mini-gels.
Gook luck and tell me now about your results!