Frank Fuerst wrote:
>> It is also possible that the kinetics of association and dissociation
> is faster than the retention time - then you'll never see dimeric or
> tetrameric peaks. This is because one molecule is and runs as a dimer
> for a fraction (depending on the equilibrium constant) of the time,
> and a tetramer for the rest. If dimers and tetramers each account for
> about half of the protein, you'll see a broad peak with the retention
> time of a trimer...
>
That was my first reaction when I read that it came out a
single peak. It may be possible to change the equilibrium
by changing the pH and/or buffer composition. Very often
this kind of rapid equilibrium is mediated by charged
species, therefore changing the pH can shift the
equilibrium. There are a number of papers on that, so do a
paper search.
> Yours, Frank
> --
> Die Verwendung von mehreren Ausrufezeichen macht die Aussage nicht
> ausrufender sondern ausufernder. [Michael Bauer in dnq]
