Hallo everybody!
I'm working on a protein that likely forms tetramers and dimers in
equilibrium. The monomer molecular mass is around 12. I would like to move
the equilibrium towards the dimers or tetramer but I have many difficult by
gel filtration to obtain the separation of the two species: tipically on a
Superose12 the sample elute in the trimer position instead of having two
defined peaks. I've also tried to use a S-100 resin but results are not
always clear. Now I'm trying a longer and sharper coloumn, but I heard
about blue native PAGE as a system to resolve different species in
solution. Anyone has experience in that and could give some suggestion? I
don't know anything about this tecnique.
Thank you very much
Valeria
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