I'm trying to strip a nitrocellulose membrane [10 mM b-mercaptoethanol, 0.1%
SDS, 20 mM Tris, pH 6.5] to restain it with another antibody . The signals
I 'm looking for are phosphoproteins. I appear to be losing more signal than
I would expect. Is there a chance that I'm hydrolyzing the phosphate group
during stripping? How do I avoid this? Thanks.