Somebody on this list once suggested to me to increase NaCl up to 0.7 M
when deglycosylating with EndoF in order to avoid precipitation of my
glycoprotein. I would like to know if Sialidases are also resistant to
the high salt concentration.
P.S. I am using a combination of EndoF2 and Sialidase C in 250 mM Na
Acetate buffer pH 5
Thank you
James
--
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* Dr. James Fethiere *
* MPI-Medical Research Int'l Tel: +49-6221-486154 *
* Ion Channel Structure Group Int'l Fax: +49-6221-486437 *
* Jahnstrasse 29 email: james at mpimf-heidelberg.mpg.de *
* 69120, Heidelberg *
* Germany *
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