Stoyan Smoukov wrote:
>> I've been working on my thesis for a couple of years. One of the goals
> is to map the interface bewteen two weakly-bound proteins. I know there
> are some methods out there like NMR mapping, FRET, but is there any
> other approaches?
Exhaustive mutagenesis (e.g. alanine scanning) is a common
approach to defining the binding interface and
characterising the binding interaction. See for example
Cunningham BC, Wells JA
J Mol Biol 1993 Dec 5;234(3):554-63
S. J. Davis, et al
PNAS 1998 95: 5490-5494.