Methods for protein-protein interface mapping

ChenHA hzhen at freeuk.com
Mon May 1 17:12:37 EST 2000

Stoyan Smoukov wrote:
> I've been working on my thesis for a couple of years. One of the goals
> is to map the interface bewteen two weakly-bound proteins. I know there
> are some methods out there like NMR mapping, FRET, but is there any
> other approaches?

Exhaustive mutagenesis (e.g. alanine scanning) is a common
approach to defining the binding interface and
characterising the binding interaction. See for example

Cunningham BC, Wells JA 
J Mol Biol 1993 Dec 5;234(3):554-63

S. J. Davis, et al
PNAS 1998 95: 5490-5494.

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