help wanted in solving a proteome problem

[sachin_16 at my-deja.com]

Thanx chris
The one that I am uysing is from Pharmacia
I am using dehydrated IEF gels which are rehydrated in solution(it has
CHAPS biolyte3-10urea andDTE.The samples were made in PGSK(phosphate
buffer and glucose refer to the EXPASY book protein extraction
fromYeast)
The ief run was as follows 300V for3 hrs followed by 3500V for 24 hrs

Waiting for more suggestion
Sachin


Sent via Deja.com http://www.deja.com/
Before you buy.