Hi,
I agree with ChrisLaRosa, that it would be very helpfull if
could go into more detail in giving information. Are you
running ief or nephge ? Do you prefocuse the rod gel, prior
to sample application? Do you have the same ampholyte mix in
your sanple buffer as in the gel? If you are prefocusing,
your sample could precipitate at the start. The same can
happen if you haven´t ampholytes in your sample buffer, etc
etc.
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