There are also different acrylamide mixes. Amresco has a PAGE-Plus,
which has worked very well in my hands for extending the range of
separation, and for the small proteins (~ 4kDa).. FMC's Long-Ranger is a
similar substance. You might also want to investigate Tricine SDS-PAGE.
There are a couple of methods you can find in PubMed. Novex's web site
(which I guess is now www.invitrogen.com) has some products and
references you also might find useful.
Good luck!
rich
Ben Gantner wrote:
> Hi all,
>> Does anyone have protocols or good web sites that discuss methods for
> improving resolution of SDS-PAGE? I'm trying to get separation of a
> fairly complex mixture, and I'm not getting particularly good
> resolution.
>> Along the same lines, are there opinions out there on PDA vs. bis as
> x-linkers?
>> Thanks for the help,
> Ben
--
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Cystic Fibrosis Research Center
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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